ADANA PROPOLİS ÖRNEKLERİNİN ANTİBAKTERİYEL VE ANTİFUNGAL ETKİSİNİN ARAŞTIRILMASI

Nizami Duran, Gülay Gulbol Duran, Haydar Öztaş, Birgül Özcan
1.938 767

Öz


Giriş ve Amaç: Propolis, yapısında 300’den fazla biyolojik olarak aktif bileşeni olan ve kompozisyonu ile bileşenlerinin oranları bölgeden bölgeye ve bitki türlerine bağlı olarak değişkenlik gösteren bir arı ürünüdür. Bu çalışmada Adana orijinli propolisin bazı Gram pozitif ve Gram suşlara karşı antibakteriyel, maya formundaki mantarlara karşı ise antifungal antifungal aktivitelerinin varlığı araştırılmıştır.

Gereç ve Yöntem: Çalışmada Gram pozitif suşlardan Staphylococcus aureus (ATCC 25923), Staphylococcus aureus (ATCC 29213), Staphylococcus epidermidis (ATCC 12228), Enterococcus faecalis (ATTCC 29212), Streptococcus pyogenes ve Gram negatif suşlardan Esherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Enterobacter cloacae (ATCC 13047), Proteus vulgaris, Enterobacter aerogenes ve maya formundaki mantarlardan ise Candida albicans (ATCC 90028), Candida krusei (ATCC 6258), Candida glabrata (ATCC 32554), Candida tropicalis (ATCC 22019) ve Candida parapsilosis kullanılmıştır. Antimikrobiyal aktivite çalışmaları makrodilüsyon yöntemi kullanılarak CLSI önerileri doğrultusunda yapılmıştır.

Bulgular ve Sonuç: Propolis örneklerinin Gram pozitif bakterilere karşı antibakteriyal etkileri (MIK değeri 64-1024 ug/ml), Gram negatif (MIK değeri 256-1024 ug/ml) ve maya suşlarına karşı elde edilen antimikrobiyal aktiviteden (MIK değeri 128-1024 ug/ml) daha yüksek olarak bulunmuştur. Mayalara karşı etkinliğin Gram negatif bakterilere nazaran daha yüksek olduğu saptanmıştır.


Introduction and Aim: Propolis is a hive product that contain more than 300 active components, and the chemical compositions and the rates of constituents varies to the geographical origin, and the plant source. The aim of this study was to investigate the activity of Adana propolis samples against some Gram negative and Gram positive bacteria and yeastlike fungi.

Materials and Methods: The following strains were included in the peresent study: Staphylococcus aureus (ATCC 25923), Staphylococcus aureus (ATCC 29213), Staphylococcus epidermidis (ATCC 12228), Enterococcus faecalis (ATTCC 29212), Streptococcus pyogenes, Esherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Enterobacter cloacae (ATCC 13047), Proteus vulgaris, Enterobacter aerogenes, Candida albicans (ATCC 90028), Candida krusei (ATCC 6258), Candida glabrata (ATCC 32554), Candida tropicalis (ATCC 22019) and Candida parapsilosis. The macrodilution method for antimicrobial activity studies was performed according to the procedure of the Clinical and Laboratory Standards Institute (CLSI).

Results and Conclusion: The antimicrobial activity of propolis against to Gram positive bacteria (MIC 64-1024 ug/ml) was higher both in Gram negative bacteria (MIC 256-1024 ug/ml) and yeast like fungi (MIC 128-1024 ug/ml). In addition, it was determined that the activity of propolis against to yeast like fungi more than Gram negative bacteria.


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