SPERMATOGENİK HÜCRELERDE BETA1 İNTEGRİN VE FİBRONEKTİN DAĞILIMI

Emel Nacar, Ahmet Nacar
1.292 380

Öz


Hücreler arası etkileşime aracılık eden fibronektin, hücredışı matriksin önemli bir bağlayıcı bileşenidir. Fibronektin, reseptörü olan â1 integrinlerle etkileşerek hücre adhezyonu, hücre göçü ve çoğalması gibi yaşamsal olaylara katılırlar. Bu çalışmada çeşitli gelişim dönemlerindeki testis dokularında fibronektin ve laminin reseptörü olan â1 integrin dağılımı literatür ışığında değerlendirildi. Sonuç olarak testiste fibronektinin hücre-hücre etkileşiminden çok hücre-bazal membran ve bazal membran-interstisyel doku etkileşiminde işlevsel olduğu; β1 integrinin ise daha çok hücre-hücre etkileşiminde ve dolayısıyla spermatogeneziste etkin olabileceği kanısına varıldı.

SUMMARY

Fibronectin takes place in cell-cell interaction and is an important connective component of extracellular matrix. Fibronectin interacts with its receptor, â1 integrin, and takes place in cell adhesion, migration and proliferation. In this study, we examined the distribution of fibronectin and â1 integrin of testis tissues in different developmental stages in the light of literature. As a result, we can conclude that fibronectin in testis is more functional in cell-basement membrane and basament membrane-interstitial tissue interaction than cell-cell interaction; and â1 integrin is essential in cell-cell interaction and spermatogenesis.


Tam metin:

PDF


Referanslar


Tanyolaç A. Özel Histoloji. Ankara: Yorum

Basın Yayın Sanayi, 1999.

Brown E, Dejana E. Cell to cell contact and

extracellular matrix editorial overview: Cell-cel

and cell-matrix interactions – running, jumping,

standing stil. Current Opinion in Cell Biology.

;15:505-8.

Giebel J, Löster K, Rune GM. Localization of

integrin

testis. International Journal of Andrology.

;20:3-9.

Magnanti M, Gismondi A, Gandini O, Rossi

FM, Michetti PM, Santtiemma V, Morrone S.

Integrin pattern and effect on contraction in

cultured testicular peritubular myoid cells.

American Journal of Reproductive

Immunology. 2001;45:21-7.

Shaller J, Glander, HJ, Dethloff J. Evidence of

cells in human testis. Human Repropduction.

;8:1873-8.

Gartner PL, Hiatt JL. Color Text Book of

Histology. Philadelphia: W.B. Saunders

Company, 2001.

Junqueira LC, Carnıero J, Kelley RO. Temel

Histoloji. Prof. Dr. Yener Aytekin, Doç.

Dr. Seyhun SolakoğluÇeviren. 8. Baskı, İstanbul:

Barış Kitabevi, 1998.

Erbengi T. Histoloji II. Ankara: Güneş

Kitabevi, 1990.

Ross MH, Kaye GI, Pawlina, W. Histology,

A Text and Atlas. Philadelphia: Williams &

Wilkins, A Wolters Kluwer Company, 2003.

Skinner MK. Cell-Cell Interactions in the testis.

Endocrine reviews. 1991;12: 45-77.

Garcia AJ, Boettiger D. Integrin- fibronectin

interactions at the cell-material interface:

initial integrin binding and signaling.

Biomaterials. 1999;20: 2427-33.

Iczkowski KA, Sun EL, Gondos B.

Morhometric study of the prepubretal rabbit

testis:germ cell number and seminiferous

tubule dimensions. The American Journal of

Anatomy. 1991;190:266-72.

Karaöz E. Özel Histoloji. Isparta: SDÜ Yayın

Evi, 2002.

Alper D, Erdem F. Adhezyon molekülleri.

Turkiye Klinikleri J Med Sci. 1997;17:75-7.

Flier A, Sonnenber, GA. Function and

interaction of integrins. Cell Tissue Res.

;305:285-8.

Hynes, RO. Cell Adhesion: old and new

questions. Trends Cell Bio. 1999;12:33-7.

Richardson LL, Kleinman HK, Dym M. The

effects of aging on basement membrane in the

testis. J Androl. 1995;16:118-26.

Schaller MD, Parson JT. Focal adhesion kinase

and associated proteins. Curr Opin Cell Biol.

;6:705-10.

Chen M S, Almeida EA, Huovila AP,

Takahashi Y, Shaw LM, Mecurio, AM. White

JM. Evidence that distinct states of the integrin

α6β1 interact with laminin and an ADAM. J

Cell Biol. 1999;144:549-61.

Salanova M, Ricci G, Boitani C, Stefanini M,

De Grossi S, Palombi F. Junctional contacts

between Sertoli cells in normal and

spermatogenic rat seminiferous epithelium

contain α6β1 integrin in their formation in

controlled by follicle-stimulating hormone. Biol

Reprod. 1998;58:371-8.

Salanova M, Ricci G, Boitani C, Stefanini M,

De Grossi S, Palombi F. Junctional contacts

between Sertoli cells in normal and

spermatogenic rat seminiferous epithelium

contain α6β1 integrin in their formation in

controlled by follicle-stimulating hormone. Biol

Reprod. 1998;58:371-8.

Palombi F, Salanova M, Taron G, Garini D,

Stefanini M. Distribution of β1-integrin sub

unit in rat seminiferous epithelium. Biol

Reprod. 1992;47:1173-82.

Dedhar S, Hannigan G. Integrin cytoplasmic

interactions and bidirectional transmembrane

signaling. Curr Opin Cell Biol. 1996;8:657-69.

Hannigan GE, Leung-Hagesteijn C, Fitz GL,

Coppolino M., Radeva G, Filmus J, Bell J,

Dedhar S. Regulation of cell adhesion and

anchorage-dependent growth by a new B1

integrin-linked protein kinase. Nature.

;379:91-6.

Ilic D, Damsky C, Yamamoto T. Focal

adhesion kinase: at the cross-roads of signal

transduction. J Cell Sci. 1997:110;401-7.

Mulholland DJ, Dedhar S, Vogl AW. Rat

Seminiferous Epithelium Conttains a Unique

(Ectoplasmic Specialization) with Signaling

Properties Both of Cell/Cell and Cell/Matrix

Junctions. Biology of Reproduction.

;64:396-407.

Giebel J, Löster K, Rune GM. Localization of

integrin β1, α1, α5 and α9 subunits in the rat

testis. International Journal of Andrology.

;20: 3-9.

Rune GM, Pretzer D, De Souza P, Bollman U,

Merker HJ. Ultrasturucture of adult juvenile

marmoset (callitrix jacchus) Sertoli cells in vivo

and in vitro. Journal of Andrology.

;13:560-70.

Suarez-Quıan CA, Hadley, MA, Dym M.

Effect of substrate on shape of Sertoli cells in

vitro. Annals of the New York Academy of

Sciences. 1985;438:417-34.

Mather JP, Wolpe SD, Gunsalus GL, Bardin

CW, Philips DM. Effect of purified and cellproduced

extracellular matrix components on

Sertoli cells functions. Annals of the New York

Academy of Sciences. 1984;438:572-5.

Glander HJ, Herrman K, Haustein UF. The

equatorial fibronektin band on human

spermatozoa: a diagnostic help for male

fetility?. Androlog. 1987;19:456-9.

Trubner M, Glander HJ, Shaller J.

Localization of adhesion molecules on human

spermatozoa by fluorescence microscopy.

Andrologia. 1997;29:253-60.

Yoshimura Y, Shiokawa S, Nagamatsu S, Sawa

H, Hanashi H, Oda T. Effect of beta 1 integrins

in the process of implantation. Horm Res.

;44:36-41.

Lessey BA, Damjanovich L, Coutifaris C,

Castelbaum AJ, Albelda SM, Buck CA.

Integrin adhesion molecules in human

endometrium: correlation with the normal and

abnormal menstrel cycle. J Clin Invest.

;90:188-95.

Hu Alpin JD. Adhesion molecules

implantation. Rev Reprod. 1997;2:4-97.

Klentzeris LD, Bulmer JN, Trejdosiewicz LK,

Morrison L, Cooke ID. Beta 1 integrin cell

adhesion molecules in the endometrium of

fertile and infertile woman. Hum Reprod.

;8:1223-30.

Miranda PV, Tezon, JC. Characterization of

fibronectin as a marker for human epididymal

sperm maturation. Mol Reprod Dev.

;33:443-50.

Sahin E, Petrunkina AM, Ekhlasi-Hundrieser

M, Hettel C, Waberski D, Harrison RA,

Töpfer-Petersen E. Fibronectin type II-module

proteins in the bovine genital tract and their

putative role in cell volume control during

sperm maturation. Reprod Fertil Dev.

;21(3):479-88.

Hencel R, Schaller J, Glander HJ, Schill

WB. Low expression of adhesion molecules

and matrix proteins in patients showing poor

penetration in zona-free hamster oocytes. Mol

Hum Reprod. 1996;2:335-9.

Glander HJ, Shaller J, Weber, W, Alexander

H, Haake KW. In vitro fertilization: increased

VLA (Very Late Antigen) integrins and

fibronectin after acrosome reaction. Archives

of Andrology. 1996;36:177-85.

Morales E, Horn R, Pastor LM, Santamaria L,

Pallares J, Zuasti A, Ferrer C, Canteras M.

Involution of seminiferous tubules in aged

hamsters: an ultrastructural,

immunohistochemical and quantitative

morphological study. Histol Histopathol.

;19: 445-55.

Santamaria L, Martin R, Codesal J, Ramirez

R, Paniagua R. Immunohistochemical

quantitative study of the peritubular lamina

propria after induction of testicular atrophy

induced by epinephrine. Int J Androl.

;18:295-306.

Gülkesen KH, Erdoğru T, Sargin CF,

Karpuzoğlu G. Expression of extracellular

matrix proteins and vimentin in testes of

azoospermic man: an immunohistochemical

and morphometric study. Asian J Androl.

;4:55-60.




Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.